Russian Journal of Developmental BiologyRussian Journal of Developmental Biology0475-14503034-6266Russian Academy of Science10.31857/S0475145023010044Modelling of the Decidualization of Mouse Endometrial Stromal Cells with Subsequent Embryo Implantation in vitroМоделирование децидуализации клеток стромы эндометрия мыши с последующей имплантацией эмбриона in vitroGaidamakaA. O.ГайдамакаА. О. stadtrand@yandex.ruИнститут биологии развития им. Н.К. Кольцова РАНKoltzov Institute of Developmental Biology of the Russian Academy of Sciences010120235415978

The decidualization of stromal cells is a key step in acquiring endometrial receptivity to the embryo. This process is regulated by sex steroid hormones, and in the mouse is additionally induced by embryo attachment. There are many protocols for the induction decidualization of stroma in vitro, but most of them require verification of the compliance of the processes occurring in cell culture with those in a living organism. This work focuses on the morphology of stromal cells and the expression activity of genes responsible for the progression of decidualization in 2D culture with subsequent embryo attachment. The feasibility of using several variants of protocols for the induction of decidualization and the possibility of using stromal cells after cryopreservation were also investigated.

Децидуализация клеток стромы – ключевой этап приобретения рецептивности эндометрия к эмбриону. Этот процесс регулируется половыми стероидными гормонами, а у мыши дополнительно индуцируется прикреплением эмбриона. В настоящее время существует множество протоколов индукции децидуализации стромы in vitro, однако большинство из них требует проверки соответствия процессов, происходящих в культуре клеток, таковым в живом организме. Эта работа посвящена изучению морфологии клеток стромы и экспрессионной активности генов, ответственных за прогрессирование децидуализации в 2D культуре с последующим прикреплением эмбриона. Также исследована целесообразность использования нескольких вариантов протоколов индукции децидуализации и возможность применения клеток стромы после криоконсервации.

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