- PII
- 10.31857/S0475145023060046-1
- DOI
- 10.31857/S0475145023060046
- Publication type
- Status
- Published
- Authors
- Volume/ Edition
- Volume 54 / Issue number 6
- Pages
- 405-414
- Abstract
- The deletions and mutations in the UBE2A gene cause X-linked mental retardation syndrome of Nascimento type first described in 2006 (Nascimento et al., 2006). To study the role of the UBE2A gene in neurodevelopment, we generated a human iPSC line with knockout of the UBE2A gene (RCPCMi009-A-1) using genome editing CRISPR/Cas9 technology. The knockout of the UBE2A gene was confirmed by Western blotting. The pluripotent state of the RCPCMi009-A-1 iPSCs line was confirmed by typical stem cell morphology, normal male karyotype maintenance, expression of pluripotency markers and the ability to differentiate into three germ layers.
- Keywords
- Х-сцепленная умственная отсталость по типу Насименто индуцированные плюрипотентные стволовые клетки ген <i>UBE2A</i> нокаут
- Date of publication
- 18.09.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 13
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